Name of the equipment: High Performance Liquid Chromatography (HPLC) Make & Model: Shimadzu, LC-20AD I-Stem Registration ID: 3245407 Category of Instrument: Chromatography Types of Analysis / Testing: Quantification Application: Identification & quantitation Description of Instrument HPLC is used to separate, identify & quantify the components in the mixture by passing through a column at a high pressure of about 400atm.
Book through I-STEM: https://www.istem.gov.in/ Slot Booking Link I-STEM Slot Booking link for External User	Booking available for Internal and External Both Requisition form for Internals Externals

Faculty In-charge: Dr. M. Sankaralingam Email ID: msankaralingam@nitc.ac.in Phone number: +91-9003586778	Technical Staff: Ruther Tyson Lewis Email ID: ruthertl@nitc.ac.in Phone number: 9845965130	Department Chemistry Office Email ID: chyoffice@nitc.ac.in Location Sophisticated Instruments Lab, Dept. of Chemistry

Features of the equipment · Pulse free solvent delivery. · Both high pressure & low pressure mixing. · Offers dual wavelength mode for detection.	Unique features/Measurement capabilities, if any
Instrument Technical Description and Major Specifications (This Specifications Limited to Major 5) · Detector: D2 lamp based UV detector. · Wavelength range: 190nm to 700nm. · Noise: 0.5×10^-5AU · Linearity: 2.5AU. · Column: C18, 250mm×4.6mm with particle size of 5µm. · pH range: 1-10 · Max pressure: 40MPa · Flow rate: 0.0001mL/min to 10.00 mL/min.	Measurement/Sample specifications:

· Quantity: 5-10 mg

· Sample Type: Solid & Liquid.

· User has to provide the standards for the quantitation. The sample has to dissolve in one of the three solvents (water, acetonitrile, methanol).

· The User should reveal the toxicity/ hazardous nature of the sample in the requisition form.

· Minimum No. of Days Required for Analysis: 1 week

	Solid/Liquid samples
NIT Calicut User	Rs. 100
External Academia Research institute User	Rs. 300
National lab User	Rs. 400
Industry User	Rs. 600