Name of the equipment: UV Visible Spectrophotometer Make & Model: Perkin Elmer Lambda 35 I-Stem Registration ID- ………………3220813   Category of Instrument Analytical Chemistry Types of Analysis / Testing ● Absorbance & transmittance measurements ● Quantitative analysis of solutions Application: ● Water quality analysis (nitrates, phosphates, heavy metals) ● Chemical concentration determination ● Environmental pollutant monitoring ● Pharmaceutical & biochemical assays Description of Instrument A double-beam UV/VIS spectrophotometer for accurate optical measurements in environmental, chemical, and biological research.

Book through I-STEM: https://www.istem.gov.in/ Slot Booking Link I-STEM Slot Booking link for External User

Booking available for Internal and External Both Requisition form for Internals Externals

Faculty In-charge: Dr. Anantha Singh T S Dr. Bhaskar S Email ID: singh87@nitc.ac.in bhaskars@nitc.ac.in Phone number: Anantha Singh: +91-8758790511 Bhaskar S: +91-9742720843

Technical Staff: Fashid V C (TA) fashidvc@nitc.ac.in Amardeep KV (Technician) amardipkumarv@nitc.ac.in

Department CED Office Email ID cedoffice@nitc.ac.in Location Environmental Engineering Laboratory, Department of Civil Engineering Lab Phone No 0495 228 6247

Features of the equipment ✔ Double-beam optics for high stability & accuracy ✔ Wavelength range: 190–1100 nm ✔ Large sample compartment for various cuvette sizes ✔ User-friendly UV WinLab software for data analysis ✔ Automatic wavelength calibration & validation	Unique features/Measurement capabilities, if any ⮚ High photometric accuracy (±0.003 A at 1 A) ⮚ Programmable scanning speeds (5–3000 nm/min) ⮚ Built-in diagnostics for performance verification
Instrument Technical Description and Major Specifications (This Specifications Limited to Major 5) ▪ Wavelength Range: 190–1100 nm ▪ Bandwidth: 1 nm (fixed) ▪ Photometric Accuracy: ±0.003 A (at 1 A) ▪ Light Source: Tungsten halogen (VIS) & Deuterium lamp (UV) ▪ Detector Type: Silicon photodiode	Measurement/Sample specifications: ▪ Sample Type: Liquid (aqueous/organic solutions) ▪ Cuvette Compatibility: 10 mm standard (quartz/glass/plastic) ▪ Absorbance Range: 0–3 A (recommended: 0.1–1.5 A for best accuracy) ▪ Concentration Range: Must be within Beer-Lambert law linearity

● Quantity: 2–3 mL (minimum for cuvette filling)

● State: Liquid (homogeneous, no bubbles/particulates)

● Pre-Preparation:

o Filter turbid samples

o Degas if bubbly

o Solvent Matched Blank Required

o pH adjustment may be needed for certain analytes

● Type of Samples Analyzed: Solutions (e.g., DNA, proteins, dyes)

● Provide solvent blanks for baseline correction.

● Label samples clearly with concentration/solvent details.

● Avoid overloading absorbance (>1.5 A may require dilution).

● Maximum Samples per Run: 1 (manual)

● Minimum Analysis Time: 1–5 minutes per sample.

Internal	External Academic Institutes	National R&D Lab	Industry
500/- per hour	1500/- per hour	1500/- per hour	3000/- per hour