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Name
of the equipment:
High-Performance
Liquid Chromatography (HPLC) System
Make
& Model:
Shimadzu
Prominence HPLC System
I-Stem
Registration ID-
3220814
Category
of Instrument
Analytical
Chemistry
Types
of Analysis / Testing
● Quantitative and
qualitative analysis of compounds
● High-performance liquid
chromatography (HPLC) separations
Application:
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Pharmaceutical
analysis
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Environmental
monitoring
● Food
& beverage testing
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Biochemical
research
Description
of Instrument
●
Modular
HPLC system for precise and reproducible separations
●
Ideal
for research, QC, and routine analysis in academic, industrial, and
clinical labs.
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Features
of the equipment
✔ Binary Pump (LC-20AD) – High-pressure
mixing with precise flow control (0.001–10.000 mL/min)
✔ Degassing Unit
(DGU-20A5R) – Online degassing for stable baselines
✔ Diode Array Detector
(SPD-M20A) – Wide wavelength range (190–800 nm) for multi-component
analysis
✔ Column Oven (CTO-10AS
VP) –
Precise temperature control (5–85°C) for improved separation
✔ LabSolutions Software – User-friendly
interface for method setup, data acquisition, and analysis
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Unique features/Measurement
capabilities, if any
⮚
High
sensitivity detection (low noise levels for trace analysis)
⮚
Wavelength
programmability for optimal peak resolution
⮚ Auto-validation and
compliance-ready (21 CFR Part 11 optional) Mass Range (MS): 1.2 – 1100
amu
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Instrument
Technical Description and Major Specifications
(This
Specifications Limited to Major 5)
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Flow
Rate Range: 0.001–10.000 mL/min
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Pressure
Range: 0–40
MPa (400 bar)
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Wavelength
Range (DAD): 190–800 nm
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Temperature
Range (Column Oven): 5–85°C (±0.1°C accuracy)
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Injection
Volume: 0.1–100 µL (manual/injection loop)
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Measurement/Sample
specifications:
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Sample
State: Liquid
(filtered, 0.2–0.45 µm recommended)
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Solvent
Compatibility: Water, organic solvents (ACN, MeOH, etc.)
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Sample
Volume Required: Typically, 10–100 µL (depends on method)
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Maximum
Sample Concentration: ≤10 mg/mL (to avoid column overloading)
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Sample State:
o Liquid (must be fully dissolved, no
particulates)
o Filtered (0.2–0.45 µm syringe
filter recommended)
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Quantity:
o Minimum volume: 0.5 mL (for
multiple injections)
o Ideal volume: 1–2 mL (for method
development/repeat analysis)
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Pre-Preparation
Requirements:
o Solvent compatibility: Must be
soluble in HPLC-grade solvents (e.g., water, acetonitrile, methanol)
o Concentration range: ≤10
mg/mL (to prevent column overloading)
o Stability: Chemically stable under
analytical conditions (specify if light-/heat-sensitive)
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Type of Samples
Analyzed:
o Small organic molecules,
pharmaceuticals, peptides, natural products, environmental pollutants
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Submission
Protocol:
o Clearly label samples with:
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Sample name/ID
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Solvent used
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Expected
concentration (if known)
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Documentation:
Provide a datasheet specifying:
o Target analyte(s)
o Preferred detection wavelength (if
applicable)
o Special requirements (e.g.,
gradient elution, column type)
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User
Instructions:
o For manual injection: Deliver samples
in HPLC vials with crimp/septum caps.
o Hazardous samples: Notify staff in
advance (e.g., toxic/corrosive compounds).
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Prohibited:
o Particulate-containing samples
(unfiltered)
o Highly viscous samples (may clog
system)
o Non-volatile buffers (e.g., phosphates)
without prior approval
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Turnaround
Time:
o Routine analysis: 1–2 days
(standard methods)
o Method development/validation: 3–5
days (requires consultation)
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Maximum Samples
per Batch: 1 sample per run
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Internal
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External Academic Institutes
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National R&D Lab
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Industry
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