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Name
of the equipment:
Fourier
Transform Infrared Spectrometer
Make
& Model:
Perkin
Elmer Spectrum 2
I-Stem
Registration ID-
…………………………
Category
of Instrument
Analytical
Chemistry
Types
of Analysis / Testing
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Material
Identification
●
Functional
Group Analysis
●
Quantitative
and Qualitative Chemical Characterisation
Application:
●
Pollutant
Analysis
●
Polymer
Degradation Studies
●
Soil
and Water Quality Assessment
●
Pharmaceutical
and industrial Quality Control
Description
of Instrument
A
Fourier Transform Infrared Spectrometer for rapid, non-destructive chemical
analysis of solids, liquids, and gases.
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Features
of the equipment
✔ High-sensitivity
DLATGS (deuterated L-Alanine doped triglycin Sulfate) detector for accurate
spectral data
✔ Universal ATR
(Attenuated Total Reflectance) accessory for solid/liquid samples
✔ Sealed and desiccated
optics for moisture-sensitive measurements
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Unique
features/Measurement capabilities, if any
⮚
Spectral
range:
4,000–400 cm-1
⮚
Resolution: Up to 0.5 cm-1
⮚
Auto-alignment
and real-time diagnostics for consistent performance
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Instrument
Technical Description and Major Specifications
(This
Specifications Limited to Major 5)
▪
Spectral
Range:
4,000–400 cm-1
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Resolution: 0.5–4 cm-1
(adjustable)
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Detector: DLATGS (standard),
optional liquid N2-cooled MCT for enhanced sensitivity
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Beam
Splitter: KBr for mid-IR range
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Supported
File Formats: .spa, .csv, .jcamp
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Measurement/Sample
specifications:
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Sample
Type
Solid (powder/film), liquid, gel
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Sample
Quantity
Solids: 1-10mg
Liquids: Thin Film (µL volume)
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Pre
Preparation
Solids:
Dry Finely Ground (for KBr Pellets)
Liquids: Free of Bubbles/Suspended Particles
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Restrictions
No Corrosive or Highly Volatile solvents
Samples must be IR transparent in measure range
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- Liquids
○ Quantity: 5–20 µL (thin film for
ATR) or 1–2 drops for liquid cells.
○ State: Homogeneous, free of
bubbles/suspended particles.
○ Pre-Preparation:
i.
Volatile
liquids: Use sealed cells to prevent evaporation.
ii.
Viscous
liquids: Apply directly to ATR crystal and ensure even coverage.
- Solids
○ Quantity: 1–10 mg (for KBr pellets)
or thin films for ATR.
○ State: Dry, finely ground powder
(particle size <2 µm to reduce scattering).
○ Pre-Preparation:
i.
KBr Pellets:
Mix 0.2–1% sample with anhydrous KBr; press at 8–10 tons.
ii.
ATR: Flatten
solid against crystal (e.g., with pressure clamp).
- Gases
○ Quantity: 10–50 mL (sealed gas cell
required).
○ State: Dry, non-corrosive.
- Restrictions:
Avoid samples containing water (interferes with IR bands) and corrosive
substances (e.g., strong acids) that damage optics.
- General
Instructions
○ Labelling: Clearly mark samples
with Name/ID, Solvent used (if applicable), Expected functional groups (e.g.,
"suspected carbonyl peak").
○ Containers: Use clean glass vials
or airtight containers for hygroscopic samples.
- Liquid
Samples
○ ATR Method:
i.
Place a drop on
the ATR crystal.
ii.
Lower the
pressure arm evenly to form a thin film.
iii.
Wipe crystal
with methylene chloride → ethanol post-use.
○ Transmission Cells: Ensure spacer
thickness matches solvent (e.g., 0.1 mm for organic solvents).
- Solid
Samples (KBr Pellets)
○ Grinding:
i.
Mix 1–2 mg
sample + 100 mg dry KBr in a mortar.
ii.
Grind briefly (over-grinding
absorbs humidity).
○ Pressing:
i.
Load mixture
into pellet die.
ii.
Press at 8–10
tons for 1–2 minutes.
○ Storage: Keep pellets in a
desiccator until analysis.
- Cleaning
Protocols
○ ATR Crystal: Wipe with solvent
(acetone → ethanol) and polish if scratched.
○ KBr Plates: Clean with methylene
chloride, then dry in the oven (100°C).
- Turnaround
& Limits
○ Max Samples/Batch: 10.
○ Analysis Time: 1–2 days (priority
scheduling available).
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Internal
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External Academic Institutes
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National R&D Lab
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Industry
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300/- per sample
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600/- per sample
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600/- per sample
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1200/- per sample
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