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Faculty
In-charge:
Dr. Md.
Anaul Kabir
Email
ID:
anaulk@nitc.ac.in
Phone number:
+91
495 228 5461
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Technical
Staff:
Sandhyadevi
S M (TA) sandhyadevism@nitc.ac.in
Sooraj
V S (ST)
soorajvs@nitc.ac.in
Jishnu
Sudhakaran (TE) jishnusudhakaran@nitc.ac.in
Sruthi K Balan (LA)
sruthikbalan@nitc.ac.in
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Department
BSED
Office
Email ID
sbtoffice@nitc.ac.in
Location
Molecular
biology lab, Bioscience and
Engineering
Department
Lab
Phone No
04952285462
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Features
of the equipment
ü The Bio-Rad CFX96™ Optics Module
is a high-performance real-time PCR detection system featuring six-target multiplexing
capability with precise thermal control and sensitive optical detection.
Supports 96-well plate format for simultaneous analysis of multiple
samples. Fast thermal cycling with accurate temperature uniformity.
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Unique
features/Measurement capabilities, if any
Ø Six-target multiplexing
capability allowing simultaneous detection of up to six different
fluorophores. Solid-state optical system with six filtered LEDs and six
filtered photodiodes for high sensitivity and specificity.Minimal
cross-talk and superior signal-to-noise ratio for precise quantification.
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Instrument
Technical Description and Major Specifications (This Specifications Limited
to Major 5)
·
Real time PCR
works on principle of cDNA as template and amplifies it with suitable
primers, added with florescent dye for real time amplification data,
amplicon not more than 250bp. Comparative multiple gene fold/expression
change analysis
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Measurement/Sample
specifications:
§ Compatible with standard 96-well
PCR plates and a wide range of fluorescent dyes (e.g., FAM, HEX, ROX, Cy5,
etc.). Detects as few as 1 copy of target nucleic acid depending on assay
sensitivity. Suitable for gene expression analysis, genotyping, mutation
detection, and pathogen quantification.
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