Name of the equipment: Microvolume spectrophotometer Make & Model: Thermo scientic ; Nanodrop One I-Stem Registration ID- 3246252 Category of Instrument Bioscience and Technology Types of Analysis / Testing Analysis Application: To measure the concentration of DNA, RNA and Protein in a 2 µL drop Description of Instrument Spectrophotometric analysis of various samples

Book through I-STEM: https://www.istem.gov.in/ Slot Booking Link I-STEM Slot Booking link for External User

Booking available for Internal and External Both Requisition form for Internals Externals

Faculty In-charge: Dr. Baiju G Nair Email ID: bgnair@nitc.ac.in Phone number:  +91-7034011575

Technical Staff: Sandhyadevi S M (TA) sandhyadevism@nitc.ac.in Sooraj V S (ST soorajvs@nitc.ac.in Jishnu Sudhakaran (TE) jishnusudhakaran@nitc.ac.in Sruthi K Balan (LA) sruthikbalan@nitc.ac.in

Department BSED Office Email ID sbtoffice@nitc.ac.in Location Room No. 307D, Department of Bioscience and Engineering Lab Phone No 04952285490

Features of the equipment ü Used to measure DNA, RNA, Proteins and other spectrophotometric readings. ü Advance sample intelligence by analyzing absorbance across full spectrum. ü Direct DNA and RNA concentration determination.	Unique features/Measurement capabilities, if any Ø Minimum sample volume required: 1-2 µl Ø Can measure upto 27500 ng/µl dsDNA and 400mg/ml of IgG Ø Spectral range: 190-850 nm | Purity ratio: A260/A280 & A260/A230
Instrument Technical Description and Major Specifications (This Specifications Limited to Major 5) § Sampling Options: Pedestal § Sample Volume: 1-2 µL § Dynamic Range: 2.0-27,500* ng/µL Spectrum: 200-800 nm	Measurement/Sample specifications: § Minimum volume Required: 5 µl (For DNA, RNA and protein estimation) § Should provide standards for protein estimation (Lowry, Bradford, BCA) § The compound should be dissolved or dispersed uniformly in water-based solvents

· Sample Type: Liquid

· Quantity: 10µL

· Preparation: Should be prepared in water or buffers

· User Instructions: Samples prepared in organic solvents cannot be used

Internal	External Academic Institutes	National R&D Lab	Industry
100/- per sample	200/- per sample	200/- per sample	300/- per sample