Name of the equipment: Inverted Fluorescent Microscope Make & Model: NIKON, Eclipse- Ti FL549274 I-Stem Registration ID- 3246255 Category of Instrument Bioscience and Technology Types of Analysis / Testing Analysis Application: Observation of fluorescently labelled fixed Mammalian cells and microbial cells. The specimen should be transparent. Non-transparent cannot be observed and imaged Description of Instrument Inverted fluorescence microscope attached with a CCD camera for taking the fluorescence images of the Biological samples

Book through I-STEM: https://www.istem.gov.in/ Slot Booking Link I-STEM Slot Booking link for External User

Booking available for Internal and External Both Requisition form for Internals Externals

Faculty In-charge: Dr. Rathinasamy K 04952285455 Email ID: rathin@nitc.ac.in

Technical Staff: Sandhyadevi S M (TA) sandhyadevism@nitc.ac.in Sooraj V S (ST soorajvs@nitc.ac.in Jishnu Sudhakaran (TE) jishnusudhakaran@nitc.ac.in Sruthi K Balan (LA) sruthikbalan@nitc.ac.in

Department BSED Office Email ID sbtoffice@nitc.ac.in Location Downstream Process Laboratory Department of Bioscience and Engineering Lab Phone No 04952285457

Features of the equipment Inverted fluorescence microscope Motorized stage Light source: High pressure Mercury Lamp Excitation filters : UV,  Blue and Green light Emission filters: Blue, Green and Red fluorescence Objectives: 40x, 20x and 5x Attached with a camera for taking images	Unique features/Measurement capabilities, if any Ø Resolution in the optical range > 25 micrometer
Instrument Technical Description and Major Specifications (This Specifications Limited to Major 5) § Inverted fluorescence microscope § Motorized stage § Light source: High pressure Mercury Lamp § Objectives: 40x, 20x and 5x Attached with a camera for taking images	Measurement/Sample specifications: § Biological sample fixed on a glass slide. The sample should be stained with a fluorophore or a fluorophore conjugated antibody.

· Biological sample fixed on a glass slide. The sample should be stained with a fluorophore

or a fluorophore conjugated antibody. Samples will be charged depending on the

number of minutes/hours used.

Internal	External Academic Institutes	National R&D Lab	Industry
Rs. 100 (per hour)	200 + 18%GST Rs. 236  (per hour)	200 + 18%GST Rs. 236  (per hour)	300 + 18%GST Rs. 354  (per hour)
Minimum Charge
Rs.100	200 + 18%GST Rs. 236	200 + 18%GST Rs. 236	300 + 18%GST Rs. 354